|
KMID : 1140120100150020164
|
|
Cancer Prevention Research
2010 Volume.15 No. 2 p.164 ~ p.171
|
|
|
Apoptosis Induction of Human Lung Cancer Cells by Methyl Alcohol Extracts of Hizikia fusiforme
|
|
Park Shin-Hyung
Shin Dong-Yeok
Eom Hyun-Sup
Chi Gyoo-Yong
Choi Yung-Hyun
|
|
|
Abstract
|
|
|
|
Hizikia fusiforme, a brown alga, has been reported to possess a wide range of beneficial activities including immuno-modulating, anti-oxidants, anti-coagulants, and anti-carcinogenic effects. According to recent studies, H. fusiforme can inhibit the growth of cancer cell by inducing apoptosis in a certain cancer lines in vitro. However, the molecular mechanism how it acts as an inducer of apoptosis is not fully understood. In this study, we investigated the apoptotic effects of H. fusiforme methyl alcohol extracts (MEHF) in A549 and NCI-H460 human lung carcinoma cell lines. It was shown that MEHF induced the cell growth inhibition in a dose-dependant manner and it was more significant in NCI-H460 cells. There also appeared increased sub-G1 phase DNA content, chromatin condensation, as well as DNA fragmentation in MEHF-treated cells, which are indicators of apoptosis. MEHF activated the caspase-8 via up-regulating Fas, FasL and DR5 expression and cleavage of Bid protein. MEHF also up-regulated the pro-apoptotic Bax levels and down-regulated anti-apoptotic Bcl-xL expression, which was associated with the loss of mitochondrial membrane potential (MMP) and activation of caspase-9 and caspase-3. Taken together, these findings suggest that H. fusiforme might be useful for clinical application to treat human lung cancer.
|
|
|
KEYWORD
|
|
|
Hizikia fusiforme, Human lung cancer, Apoptosis, Caspase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|